CYP1A1 gene polymorphism.docVIP

 PAGE \* MERGEFORMAT 13 CYP1A1 gene polymorphism Of: Liu Fang Li Hua Liu Kaiyang Sun Li Yang Cuijun [Abstract] Objective To investigate the CYP1A1 gene in healthy people in Zhangjiakou area MspI and Ile / Val polymorphism. Methods allele specific amplification and PCR restriction fragment length polymorphism (RFLP technology, analysis of 360 cases of healthy adults Zhangjiakou region the CYP1A1 gene 3 ‘MspI restriction enzyme site and Exon7 site 3 genotype. The results of Zhangjiakou MspI genotype m1m1 healthy population, 35.8%, 50.2% genotype m1m2, m2m2 genotype accounted for 14.0%, m1m2 allele were 60.9%, 39.1% .Ile / Val polymorphism and allele frequency of Ile and Val were 68.5%, 31.5% .Ile / Ile, Ile / Val, Val / Val genotypes were 44.4%, 48.1%, 7.5%. Conclusion Zhangjiakou region genes of healthy people CYP1A1 MspI and Ile / Val polymorphism. [Keywords:] CYP1A1, MspI, Ile / Val, gene polymorphism CYP1A1 is the cytochrome P (CYP) 450 family, a member of a body of important detoxifying enzymes of phase involved in a variety of endogenous and exogenous chemicals in the body metabolism (1). CYP1A1 gene encoded by the CYP1A1, CYP1A1 expression is regulated by induced genes and environmental factors, and racial and individual differences. mainly distributed in lung, kidney, gastrointestinal tract, skin, placenta and other tissues involved in endogenous and exogenous compounds in vivo metabolic processes, mainly through oxidation, reduction, hydrolysis and so on, change the compounds with the functional groups. or to break down. CYP1A1 mainly involved in polycyclic aromatic hydrocarbon compounds (PAH) metabolism, its metabolic activity is very strong phenolic epoxide, which epoxide in the carcinogenic, mutagenic important role in both (2,3). So far, CYP1A1 gene have been found MSPI and Ile / Val polymorphism in different racial, ethnic and regional distribution are very different. The testing by polymerase chain reaction and restriction fragment len