Deep hypothermic circulatory arrest microdialysis study of spinal cord injury.docVIP

 PAGE \* MERGEFORMAT 5 Deep hypothermic circulatory arrest microdialysis study of spinal cord injury Authors: LIU Ning-ning, SUN Li-zhong, ZHENG, TIAN Liang Xin, Long Village [Abstract] Objective To observe the use of microdialysis technique during deep hypothermic circulatory arrest pig spinal cord nerve cells in the interstitial fluid in a variety of substances, and to explore the molecular mechanism of spinal cord injury. Methods 6 miniature pig, was implanted in the lumbar spinal cord of two micro-dialysis needles. The establishment of cardiopulmonary bypass, parallel loop cooling for 30 min to the rectal temperature 20 ℃ , circulatory arrest 60 min, a modest recovery in parallel loop 120 min. At a fixed point in time microdialysis samples were collected to detect amino acids and energy metabolism substances, observe trends. The results during hypothermic circulatory arrest significantly decreased pyruvate, lactate / pyruvate ratio reached a peak. After rewarming of glucose, lactate, pyruvate and glycerol were significantly increased. During cooling of glutamate and γ -amino butyric acid increased significantly. Aspartic acid, taurine, and glycine increased significantly during the rewarming. Conclusions stop cycle, the energy metabolism of neurons was significantly inhibited. The rate of rewarming in some recovery of energy metabolism, cell at a high-sugar, high-acid environment. During cooling of glutamate and γ -amino butyric acid increased rewarming transit aspartic acid, glycine and taurine were significantly increased may be related to spinal cord injury. [Keywords:] spinal cord injury; deep hypothermic circulatory arrest; micro-dialysis Abstract: OBJECTIVE To investigate the changes in energy-related metabolites and the concentration of amino acid in spinal cord during deep hypothermic circulatory arrest. And discuss the mechanism of spinal cord injury. METHODS Microdialysis probe was inserted into the lumbar spinal cord of six pigs respectively