Deficiency state changes in rats Study on mechanism of cAMP.docVIP

 PAGE \* MERGEFORMAT 11 Deficiency state changes in rats Study on mechanism of cAMP Of: Li Min, Zhang Bing, Liu Xiaoqing, Jinyong Nan, Young-Fa Lai Jin, Ni Li [Abstract] Objective To observe the plasma in rats under Deficiency second messenger cyclic adenosine monophosphate (cAMP) levels, and to explore its possible mechanism. Methods Rats were randomly divided into model group and the Deficiency group, cold day group hydrocortisone intramuscular injection, intramuscular injection of normal saline per day, continued 14 d; animals were killed, three iodine thyroid hormone Thyroid original acid (T3), thyroxine (T4), anti three iodine A gland of the original acid (rT3), adrenocorticotropic hormone cortisol (COR), cyclic adenosine monophosphate (cAMP), cyclic guanosine monophosphate (cGMP), adenylyl cyclase (AC), adenosine triphosphate (ATP). Results Deficiency status of endocrine hormones T3, T4, rT3, COR lower level, cAMP levels, AC activity decreased, ATP content increased. Conclusion Deficiency state of the thyroid gland, adrenal cortex reduced the role of endocrine hormones in the AC, for AC activation and decreased production of second messenger cAMP, which mediates the biological effects of reduced accordingly, so the inhibition of Deficiency show the state of the body. [Keywords:] Deficiency state; cyclic adenosine monophosphate; adenylyl cyclase; adenosine triphosphate; endocrine hormone; rat Abstract: Objective To investigate the change of endocrine, cAMP and potential mechanism in asthenia cold syndrome rats. Method The rats were randomly divided into normal group and asthenia cold model group. The model group were treated with intramuscular injection of hydrocortisone for 14 days, and the normal group with NS. The content of cyclic adenosine monophosphate (cAMP) and adenylate cyclase (AC), triiodothyronine (T3), thyroxine (T4), dopamine (rT3), cortisol (COR), cyclic guanosine monophosphate (cGMP) was detected by radioimmunoassay (RIA). The c