Design - response surface method and orthogonal experimental design optimization process Comparison of Epimedium.docVIP

 PAGE \* MERGEFORMAT 12 Design - response surface method and orthogonal experimental design optimization process Comparison of Epimedium Of: Wang Wendi, Wang Yang, Wang Lifeng, Liu Xiaoli, Xiao-Juan Zhang [Abstract] Objective To design and orthogonal central composite design and response surface methodology experimental design of two, preferably Epimedium technology, and design methods for comparison. Way to extract the content of icariin as the dependent variable, ethanol concentration, reflux time and solvent (times) the amount as the independent variables, we chose technology. Results orthogonal experimental design to determine the optimum process is 12 times the amount of 60% ethanol extracted twice, 3 h / times; composite design response surface methodology to determine is 12 times the optimum amount of 50% ethanol and extracted twice, 160 min / times. Conclusion design - response surface methodology study of the extraction process is better than the orthogonal experimental design method for optimization of extraction process used in traditional Chinese medicine provide a basis for the feasibility. [Keywords:] orthogonal design; design - response surface methodology; icariin; extraction process Abstract: ObjectiveTo optimize the process of extacting effective constituents from Epimedii Herba by orthogonal experimental design and central composite design-response surface method.MethodsIndependent variables were concentration of ethanol, extraction time, times of reflux and solvent fold.Dependent variable was extraction rate of icariin in Epimedii Herba.Linear or nonlinear mathematic models were used to estimate the relationship between independent and dependent variables.ResultsThe result of the orthogonal experimental design was 60% ethanol, 180 minutes for reflux, 12 fold of solvent and 2 times for extraction.The other was 50% ethanol, 160 minutes for reflux, 12 fold of solvent and 2 times for extration.ConclusionThe Central Composite Design-R