Diacetyl monoxime - thiosemicarbazide method for the determination of urea in water pool.docVIP

 PAGE \* MERGEFORMAT 5 Diacetyl monoxime - thiosemicarbazide method for the determination of urea in water pool [Keywords:] diacetyl monoxime - thiosemicarbazide pool urea Urea is a product of protein metabolism in the human body, mainly by the kidneys with the urine, some can be discharged with the sweat. When more swimmers, the swimmers sweat, urine urea would increase the pool water, so lt;lt;swimming place Standardgt;gt; (GB9667-1996 to determine the subject for the evaluation of swimming pool water is an important indicator of human pollution. Urea soluble in water, with irritation. If high levels of urea in swimming pool water, swimmers will appear pruritus, flushing, erythema, scaling and other symptoms. Our country in the “lt;standard for swimming placegt;gt; in the provision of swimming pool water urea content of not more than 2.5mg / L. At present, for the determination of urea in swimming pool water method phthalaldehyde method, phenol - hypochlorite salt spectrophotometric, OPA method, the urine enzyme-Berthlot law, diacetyl monoxime method [1]. diacetyl monoxime - antipyrine method is the national standard method [2] Although the law for many years, but is still exposed in the linear range of applications, narrow, long reaction time, reaction products of instability and fugitive and other shortcomings. For this, we diacetyl monoxime - thiosemicarbazide solution alternative diacetyl monoxime and antipyrine solution to improve and stabilize the product color [3], and achieved satisfactory results. 1 Reagents and Methods 1.1 Reagents and preparation 1.1.1 Diacetyl monoxime - thiosemicarbazide solution Weigh diacetyl monoxime 600mg, thiosemicarbazide 30mg, dissolved in water and diluted to 100mL. 1.1.2 concentrated phosphoric acid solution mixed 70ml, concentrated sulfuric acid 160mL, slowly add in about 500mL water, after cooling diluted to 1 000mL. Standard stock solution of urea 1.1.3 Accurately weigh 0.100 0g urea in a small