Diagnosis of 383 cases of hemolytic disease of newborn experimental study.docVIP

 PAGE \* MERGEFORMAT 7 Diagnosis of 383 cases of hemolytic disease of newborn experimental study Of: Xu Chang-Gen, Zhu Yangquan, Lee, Lian, Qiao Hu, Jiang Lu [Abstract] Objective of the 383 cases of neonatal hemolytic disease serological testing for clinical diagnosis available. Ways censorship of 383 cases of hospital neonatal hyperbilirubinemia blood samples were “three tests”, that is directly antiglobulin test, free antibody test, release test antibody. Results in 383 cases of neonatal hemolytic disease, direct antiglobulin test positive rate was 53.12%, free antibody test positive rate was 71.54%, positive rate of antibody release test to 83.29%. Conclusion release most sensitive test is to determine the most powerful hemolytic disease of newborn laboratory evidence. [Keywords:] David polycythemia, fetal, serology, direct antiglobulin test, free antibody test, release test antibody Hemolytic disease of newborn (heamolytic disease of the newbown, HDN is the mother and child blood group incompatibility, maternal blood for fetal red blood cells IgG antibody through the placenta into the fetal circulation, the occurrence of alloimmune hemolysis caused. In human blood have been found systems to sub-ABO blood group system of neonatal hemolytic disease caused by the most common, followed by the Rh blood group system. HDN serological detection of the disease has important clinical value. author of Zhenjiang City in recent years occurred in 383 cases of HDN serological analysis of test results as follows. 1 Materials and methods 1.1 The source of specimen in January 2005 ~ December 2007 banning the city clinical hospital 4 hyperbilirubinemia or mother is Rh negative blood group in children with 383 cases of HDN, the blood samples for the children and mothers. 1.2 Reagent Anti-A (B serum (Changchun Institute of Biological Products, anti-D, anti-human globulin reagents (broad-spectrum, monoclonal IgG, monoclonal antibody C3 (Shanghai Blood Center