Diffusa Bel7402 on human hepatocellular carcinoma cell apoptosis.docVIP

 PAGE \* MERGEFORMAT 17 Diffusa Bel7402 on human hepatocellular carcinoma cell apoptosis Of: Lvxiu Fang, Meng Qingyu, Guo Xinmin [Abstract] Objective To study gene level diffusa on cultured human liver cancer cells induced apoptosis Bel7402 and its possible mechanism. Methods Bel7402 cells in normal human liver cancer in vitro, a randomized control group , 5 - fluorouracil (5-Fu) positive control group, diffusa group. inverted microscope to observe changes in cell morphology. HE staining, cell morphology observed under light microscope, calculated the apoptotic index. by MTT assay nosed pit viper tongue grass growth inhibition of cancer cells. Semi-quantitative RT-PCR Detection of Bcl-xl, p53 gene mRNA expression. Results diffusa light microscope to wall off the tumor cells, a cell budding phenomenon of morphological changes of apoptosis, apoptotic index slightly lower than 5-Fu positive control group, but compared with the control group were significantly different (P lt;0.01), inhibition rate slightly lower than 5-Fu positive control group, compared with the control group were significantly different (P lt;0.01), p53 gene mRNA expression increased, decreased Bcl-xl gene mRNA, compared with the control group were significantly different. Conclusions diffusa Bel7402 cell growth inhibition of human hepatocellular carcinoma and induce apoptosis, and its molecular mechanism may be related to activation of tumor suppressor gene p53, inhibition of oncogene Bcl-xl gene expression. [Keywords:] diffusa; human hepatoma Bel7402 cells, apoptosis, Bcl-xl gene, p53 gene Abstract: Objective To study the inducing apoptosis of Hedyotis diffusa extract (HDE) on human hepatocarcinoma cell line Bel7402 and its molecular mechanism. Methods Human hepatocarcinoma cell line Bel7402 was used in vitro cell culture, there were 3 groups: control group, HDE group and 5-Fu group, the Bel7402 cells were dealed with them respectively. The apoptosis morphologic changes of human