Doxycycline inhibits apoptosis induced by mitomycin in MTEC1 its preliminary analysis of proteins.docVIP

 PAGE \* MERGEFORMAT 17 Doxycycline inhibits apoptosis induced by mitomycin in MTEC1 its preliminary analysis of proteins Of: Shen Weihong, Liu Hui, Peng Xin, Xiao Xueyuan, He Dacheng CHEN Weifeng, Shao Qixiang [Abstract] Objective: To investigate the doxycycline (doxycycline, Dox protection of thymic epithelial cells and its mechanisms for further study of immune regulation of Dox basis. Methods: Using the optical microscope Dox of MMC (mitomycin, MMC ) MTEC1 induced apoptosis, while using Hochest33342, PI single staining, Annexin V and PI double staining confirmed its role, and finally by surface enhanced laser desorption ionization time of flight mass spectrometry analysis of the SAX2 chip capture the differences before and after Dox proteins. Results: The morphology, Hochest33342, PI single staining and double staining Annexin V and PI that can really protect the MTEC1 Dox against apoptosis induced by mitomycin. followed by surface enhanced laser desorption ionization time of flight mass spectrometry analysis of SAX2 chip capture the protein and found that Dox can control 15 types of proteins, which increased 8, down 7. CONCLUSION: Dox protect MTEC1, inhibition of apoptosis induced by MMC, and MTEC1 regulation of protein expression. [Keywords:] doxycycline; thymic medullary epithelial cell line; apoptosis, protein chips [Abstract] Objective: To expose the molecular mechanism of doxycycline (Dox) that protects the mouse thymic epithelial cell line (MTEC1) free from apoptosis which induced by mitomycin (MMC). Methods: The effect of Dox protects the MTEC1 cells anti apoptosis against the MMC induced was observed under an optical microscope, and confirmed by Hochest33342 stain, PI single stain, Annexin V and PI double stain, and observed by fluorescence microscope or flow cytometer. Furthermore, SELDI Protein Chip was used to analyze the profile proteomics of MTEC1 cell which treated by doxycycline or not. Results: Dox was found to protect the MTEC1