DPG modification activity assay Methods.docVIP

 PAGE \* MERGEFORMAT 11 DPG modification activity assay Methods [Abstract] Objective To establish a biochemical automatic analyzer for determination of phosphoglycerate dynamics mutase (phosphoglyceromutase, PGAM methods and to evaluate the reliability of this method. To further explore the human serum PGAM activity in acute myocardial infarction and cerebral hemorrhage early role in diseases such as to establish a good method. Methods PGAM activity was measured by 3 - phosphoglycerate as the substrate, after a series of enolase coupled to 340 nm wavelength measured in the automatic analyzer, according to NADH absorbance degree of change in the total PGAM activity can be obtained. The results determined through a series of parameters can be adapted to establish a good automatic biochemical analyzer PGAM assay. Conclusion automatic enzyme coupling method is simple and rapid determination, good stability, sensitivity and high precision, strong anti-interference, can be applied on automatic biochemical analyzer. [Keywords:] phosphoglycerate mutase myocardial infarction cerebral hemorrhage Kinetic Abstract: Objective To establish a method applicable to assay PGAM in an automatic biochemical analyzer dynamics and to evaluate the reliability of this method. To further explore the early role that human serum PGAM activity plays in acute myocardial infarction and cerebral hemorrhage, and other diseases and as a result find out a desirable method. Methods The activity was determined by 3 - phosphoglycerate as substrate, the enolase and a series of coupling to 340 nm wave-length automatic analyzer in the determination of total activity could be derived. Results Through a series of parameters determination, a good automatic biochemical analyzer to the PGAM assay was established.Conclusions Automatic-coupling method has the advantage of simplicity, fast-determination, good stability, high accuracy and sensitivity, and strong anti-interference, availability to the