Drug sensitivity tests to guide chemotherapy on apoptosis of gastric cancer in mice and the effects of P53 gene.docVIP

 PAGE \* MERGEFORMAT 14 Drug sensitivity tests to guide chemotherapy on apoptosis of gastric cancer in mice and the effects of P53 gene [Abstract] Objective: To test screening sensitivity through the application of multiple chemotherapy drugs on tumor-bearing mice passage of chemotherapy, to understand its efficacy as well as tumor apoptosis and P53 genes. Methods: The MTT assay of gastric cancer cells in mice 6 kinds of chemotherapy sensitivity of chemotherapy tumor-bearing mice were observed after chemotherapy, tumor size, tumor inhibition rate of apoptosis, and P53 gene expression. Results: The MTT method for screening the most sensitive to chemotherapy drug 5  FU of the first generation of chemotherapy in tumor-bearing mice and its inhibition rate, high rate of apoptosis, P53 gene expression decreased compared with the control group there was significant difference; least sensitive to the low rate of ADM antitumor chemotherapy, apoptosis rate is low, P53 gene expression in control group (P amp;lt;0.05). again has been in chemotherapy of gastric cancer cells, the most sensitive screening of drugs for the DDP, the least sensitive to drugs with the MMC, the second-generation chemotherapy in tumor-bearing mice found that inhibition rate of DDP group, the high rate of apoptosis, P53 gene expression lower than the control group (P amp;lt;0.05); MMC group inhibition rate is low, the low rate of apoptosis, P53 protein expression in high compared with the control group without significant difference. And following with the 5  FU and ADM chemotherapy on the second generation of chemotherapy in tumor-bearing mice, inhibition rate is low, the low rate of apoptosis, P53 gene expression in high compared with the control group, no significant difference. Conclusion : susceptibility testing, detection of apoptosis and P53 gene can be used as the choice of chemotherapy and prognosis indicators. [Keywords:] drug sensitivity testing of gastric tumor cell apoptotic gene