山葵叶、叶柄混合物的润肠通便作用及其功效物质的-第三军医大学学报.doc

PAGE  PAGE 10 山葵叶、叶柄混合物的润肠通便作用及相关功效物质的初步研究 刘先俊1 刘方欣1 周惠云2 1 重庆医科大学，重庆 400016；2重庆康尔威药业股份有限公司，重庆 401122 摘 要 目的：探讨山葵叶、叶柄混合物的润肠通便作用及其相关的功效物质，为进一步开发利用这一山葵种植中的副产品作为医药用途提供依据。 方法：将新鲜山葵叶、叶柄采用冷冻干燥，粉碎后获得叶片、叶柄混合物，通过酶－重量法测定了其总膳食纤维、可溶性膳食纤维与不溶性膳食纤维，并将其通过水解除去异硫氰酸盐、除去水溶性成分等。分别研究了山葵叶、叶柄混合物干粉（样品1）、除去异硫氰酸盐后（样品2）、除去水溶性成分后（样品3）等的润肠通便功效（包括小肠功能试验、首次排黑便时间、6h排便粒数与6h排便总量）。 结果：山葵叶、叶柄混合物冻干粉中总膳食纤维量为92.3g/Kg、可溶性膳食纤维（SDF）含量为48.2g/Kg，不溶性膳食纤维(IDF)含量为44.1g/Kg。SDF/IDF比值为1.1。在润肠通便功效方面，上述3种样品均有润肠通便功效且相互间比较，其差异具有显著性(P 0.01或0.05)。 结论：山葵叶与叶柄混合物中膳食纤维含量偏高且可溶性膳食纤维较多；山葵叶与叶柄混合物对复方地酚诺酯模型便秘动物有润肠通便作用；其润肠通便作用相关的功效物质多样包括异硫氰酸盐以及存在于其水溶性成分、水不溶性成分中的物质成分等。 关键词：山葵，叶，叶柄，润肠通便 中图分类号：R961;Q946.8 文献标识码：A Preliminary Study for Catharsis Effect and Its Efficiency Matter in Mixture of Leaf and Leaf stalk in Wasabi Liu Xian-jun1 , Liu Fang-xin1, Deng Xian-feng2, Zhou Hui-yun2 1 Chongqing Medical University, Chongqing 400016,China; 2Chongqing K.E.W.Pharmaceutical Co.,Ltd, Chongqing 401122,China Abstract AIM:To explore catharsis effect and its efficiency matter in mixture of leaf and leaf stalk in Wasabi, and to provide basas for development and utilization leaf and leaf stalk in Wasabi that is by-products in Wasabi planting as  HYPERLINK /dict_result.aspx?searchword=%e5%8c%bb%e8%8d%af%e7%94%a8%e9%80%94tjType=sentencestyle=t=pharmaceutical+application pharmaceutical application. Methods:The mixture of dried powder for Leaf and leaf stalk in Wasabi is obtained by freeze-drying and crushing for fresh Leaf and leaf stalk in Wasabi. Using enzymatic-gravimetric method, total dietary fibre, soluble dietary fibre(SDF) and insoluble dietary fibre(IDF) in mixture of dried powder are determined. Isothiocyanates in mixture of dried powder is removed by adding water and incubating in 37℃ overnight. The matters of soluble in water is removed by adding water, incubating in 37℃ overnight, centrifugation in 5,000rpm×10min and precipitation colleted. It is carried out the effect on moistening the intestines and freeing the stool in mi