非小细胞肺癌中Reprimo以及1433 Σ基因启动子异常甲基化检测的论文.docVIP

　　非小细胞肺癌中Reprimo以及1433 Σ基因启动子异常甲基化检测的论文 【摘要】 目的探讨reprimo以及1433 σ基因启动子在非小细胞肺癌（nsclc）中的异常甲基化状态及其与临床病理资料的联系。方法采用甲基化特异性聚合酶链反应（methylation specificpcr,msp）技术检测60例nsclc以及癌旁正常组织中reprimo以及1433 σ基因启动子异常甲基化状态。结果reprimo以及1433 σ基因启动子异常甲基化在nsclc组织中发生频率分别为36.67％(22/60)和28.33%(17/60)，与癌旁正常组织具有显著性差异（p值分别为0.000和0.002）。reprimo基因启动子异常甲基化频率与吸烟习惯以及年龄相关；1433 σ启动子异常甲基化与年龄、吸烟习惯、性别、病理类型和临床分期以及淋巴结转移等无相关。结论nsclc中存在reprimo和1433 σ等基因启动子较高频率的异常甲基化。 【关键词】 肺癌;甲基化;reprimo基因;1433 σ基因 detection of aberrant methylation of reprimo and 1433 σ genes in nonsmall cell lung cancer jiang rui，yu shiying cancer center of tongji hospital, tongji medical college, huazhong science and technology university, ail: syyu@. abstract：objective to investigate the frequency of aberrant methylation of reprimo and 1433 σ in nonsmall cell lung cancer (nsclc).methodsaberrant methylation of reprimo and 1433 σ ined by methylation specificpcr (msp) in primary nsclc and normal lung tissues (n = 60).resultsaberrant methylation of reprimo and 1433 σ ethylation of reprimo(5%) and 4 cases of 1433 σ(6.67%) al lung tissues. the frequencies of aberrant methylation of reprimo and 1433 σ betary nsclc and normal lung tissue group ethylation of reprimo ore frequently observed in older patients group (p=0.047) and smoking group (p=0.038). there o methylation frequencies and sex, pathology type, clinical staging and lymphatic metastasis. similarly no significant association ethylation frequencies and sex, age, pathology type, clinical staging, smoking habit and lymphatic metastasis.conclusionmethylation of reprimo and 1433 σ may play an important role in the pathogenesis of nsclc. key ethylation; reprimo; 1433 σ tab 1primer sequence of reprimo and 1433σgeneforerreverse primerproduct1433σmtggtagtttttatgaaaggcgtccctctaaccgcccaccacg105bp1433σuatggtagtttttatgaaaggtgttccctctaaccacccaccaca107bpreprimomgcgagtgagcgtttagttctacctaaaaccgaattcatcg112bpreprimouttgtgagtgagtgtttagtttgtaattacctaaaaccaaattcatc112