颈椎旁肌和韧带异常对颈椎退行性变影响的实验研究的论文.docVIP

　　颈椎旁肌和韧带异常对颈椎退行性变影响的实验研究的论文 颈椎旁肌和韧带异常对颈椎退行性变影响的实验研究 【关键词】 颈椎旁肌;韧带;退行性变;动物实验 【摘要】 目的 研究颈后椎旁肌和韧带异常对颈椎退行性变的影响。方法 将36只6～9个月龄新西兰大白兔随机分为实验组(20只)和对照组(16只),实验组动物切除棘上和棘间韧带,分离颈后旁两侧肌肉,造成颈椎力学上的失衡。在术后4个月和10个月时分别拍摄颈椎正侧位和过伸过屈位x线片,观察颈椎的稳定性,然后处死动物,取出c4，5、c5，6椎间盘组织,行he染色,光镜检查,在形态学上评定颈椎间盘退变程度并根据miyamoto法进行分级。结果 对照组4个月、10个月未见椎间隙狭窄、骨赘形成和序列异常。实验组4个月无骨赘形成，10个月可见椎间隙狭窄、骨赘形成。4个月2组相比出现不稳定的节段数阳性率比较差异无统计学意义(pgt;0.05),10个月时2组差异有统计学意义(plt;0.05),10个月对照组与4个月实验组相比出现不稳定的节段数阳性率无统计学意义(pgt;0.05)。组织学研究结果为对照组10个月与4个月相比随着增龄颈椎间盘退变明显(p﹤0.05)，4.10个月时2组相比退变均明显(p﹤0.05)。结论 颈椎椎旁肌及韧带的异常可以加速颈椎的退变,表现为颈椎间盘退变和颈椎不稳的出现早于自然的退变过程。 【关键词】 颈椎旁肌;韧带;退行性变;动物实验 　the experimental study on the cervical degeneration because of the anomaly of cervical posterior paravertebral muscles and suprainterspinous ligament sun xianze,shen yong,li feng,et al. department of spinal surgery,the third hospital of shijiazhuang, shijiazhuang 050000,china 　　【abstract】 objective to study the effect of anomalous paravertebral muscles and suprainterspinous ligement to cervical disc and vertebral degeneration.methods thirtysix neonths ized into tental group(e group) and 16 rabbits for the control group(c group). e group rabbits posterior paravertebral muscles the spinous, laminas and facets of the cervical vertebrea. next，the supraspinous and interspinous ligaments of the cervical vertebrea onths，anteroposterior and lateral radiographs(extebtion,neutral and flexion)ed in the 18 rabbits in e4m and c4m groups to examine disc spase narroation and changes in spinal alignment. next，the animals ens, c4.5 and c5.6 in each animal atoxylin and eosin for light microscopic examination and graded acorrding to miyamoto method. after 10 months， the others (e10m and c10m groups)ined and killed simultaneously. results in the c4m， c10m groups， no discspace narroation， or abnormal spinal alignment ns of the animals in the e4m group reveavled no evidence of osteophyte formation.in the vertebral columns of all the animals in the e10m group，hoation. there and c4m