骨髓基质干细胞治疗实验性自身免疫性脑脊髓炎的实验研究的论文.docVIP

　　骨髓基质干细胞治疗实验性自身免疫性脑脊髓炎的实验研究的论文 【摘要】 目的：通过体内、外实验，研究骨髓基质干细胞（bmscs）对实验性自身免疫性脑脊髓炎（eae）的治疗作用及其机制。方法：建立lescs，于发病高峰期处死大鼠，组织取材。通过临床评估、荧光示踪、免疫学病理学等方法检测，评估bmscs对eae的治疗作用及其机制。同时在体外用化学方法诱导bmscs向少突胶质细胞分化，观察其分化的情况。结果：通过尾静脉回输，bmscs能够聚集到病变部位，并发生分化。与对照组相比，回输组大鼠发病临床评分较低，中枢神经系统脱髓鞘的病理改变和炎性细胞浸润减轻，cd4+和cd8+t细胞表达明显减少（plt;0.05），血清及淋巴细胞培养上清中ifnγ和tnfα的分泌水平显著降低（plt;0.05），il4的分泌水平显著升高（plt;0.05）。体外诱导72小时未见到bmscs向少突胶质细胞分化；4天后45%的bmscs发生分化。结论：在体外bmscs通过化学诱导可以向少突胶质细胞分化；在体内bmscs能向病变部位迁移，并部分分化成少突胶质细胞，同时bmscs通过调节免疫系统的炎症细胞因子表达、cd4+和cd8+t细胞数和th1和th2细胞平衡，对eae起治疗作用。 【关键词】 eae bmscs 分化 细胞因子 　　study on treatment of experimental autoimmune encephalomyelitis by bone marroal cells 　　li hulun,sun bo, mu lili, liu xijun, ent of neurobiology,affiliated hospital of tongji medical college,huazhong university of science and technology,arro cells(bmscs)for the experimental autoimmune encephalomyelitis(eae)and the related mechanisms underlying,and to investigate the differentiation of bmscs under oligodendroglial cell microenvironment induction both in vivo and vitro.methods:establishing the microenvironment and stimulating bmscs in vitro. immunizing leent group, marked bmscs munization. evaluating the corresponding indexes of eae by clinical assessment, immunohistochemistry, elisa and so on, thoroughly discussing the pathogenesis and the therapeutic effects of bmscs to eae.results:72 hours after stimulating scs to differentiate to oligodendrocyte. then 4 days later,45% bmscs differentiated to oligodendrocytes. bmscs could also migrate to the spinal cord yelination occurred. by injecting bmscs into the rat tail vein,patholoqical demyelination of central nerve system and inflammatory infiltration e extent.the amount of cd4+ and cd8+t cells and the concentrations of ifnγ and tnfα in the sera significantly decreased（plt;0.05）, scs have the capacity to migrate to the injured tissues and differentiate into oligodendrocyte. bmscs may reverse the disbalance of th1/th2 cells by regulating the cytokines production