Effects of aging on splenic CD4 + CD25 + T cells.docVIP

 PAGE \* MERGEFORMAT 8 Effects of aging on splenic CD4 + CD25 + T cells Of: WANG Junlizhixin Tianhong Yan Xu Yezhuxinwei willow [Abstract] Objective To compare the young rats and aged mouse spleen CD4 + CD25 + T cell number and function changes. Methods Flow cytometry analysis to detect splenic CD4 + CD25 + T cell percentage and Foxp3 and GITR expression; doped by 3H TdR into the determination of splenic CD4 + CD25 + T cell function. Results aged spleen CD4 + CD25 + T cells significantly increased the percentage, but the suppression was significantly reduced, and reduced surface expression of GITR. Conclusion With increasing age, spleen CD4 + CD25 + T cells inhibit the function decreases. [Keywords:] CD4 + CD25 + T cells; Age; Foxp3; GITR Natural CD4 + CD25 + regulatory T cells in the immune response from inhibition to maintain the body’s own immune tolerance and foreign antigens (1,2), its development and function by Foxp3 control (3,4), its inhibitory function and Expression of cell surface molecules GITR and other related (5). In recent years, these cells the role of autoimmune diseases has attracted wide attention. autoimmune diseases common in the elderly, with increasing age, natural CD4 + CD25 + regulatory T cells is not entirely clear what has changed. In this paper, to study the aging mouse CD4 + CD25 + T cells in the changes of age on immune function. 1 Materials and methods 1.1 The selected experimental animals 3 months (young group) and 20 months (aged group) level SPF Balb / C female mice, purchased from Beijing Vital River Company. 1.2 Reagent and instrument fluorescent labeled anti-mouse CD4, CD25, GITR monoclonal antibody and antibody isotype controls (BD), fluorescence labeled anti-mouse Foxp3 mAb (eBioscience), mitomycin C (MMC, Kyowa Hakko ), 3H TdR (Beijing Atomic Energy Research Institute), flow cytometry (BD), liquid scintillation counter (LKB). 1.3 derived spleen of mice, preparation of single cell suspension, eryt