Effects of losartan and simvastatin on the kidney of diabetic rats NF-κBPDGF-B and the expression of PKCα.docVIP

 PAGE \* MERGEFORMAT 2 Effects of losartan and simvastatin on the kidney of diabetic rats NF-κBPDGF-B and the expression of PKCα 【Abstract】 Objective streptozotocin (STZ)-induced diabetic Wistar rats with diabetic control, diabetes, respectively losartan, simvastatin, as well as with the combination therapy with simvastatin and losartan on the four kinds of conditions under the renal NF-κB, PKCα, and PDGF-B expression, so as to clarify the NF-κB, PKCα, and PDGF-B in diabetic rat kidney changes and possible mechanisms of drug action. Methods of urinary albumin measured by radioimmunoassay and calculate the 12h urinary protein excretion rate (UAER), while measuring FBG, serum cholesterol, serum triglycerides, serum creatinine. HE staining of renal biopsy conventional microscopic observation of pathological changes in diabetic nephropathy. Using SABC immunohistochemical method to detect the group of glomerular, tubular NF-κB, PKCα, and PDGF-B expression of the three proteins. With image acquisition and analysis software for immunohistochemical staining to conduct quantitative analysis. Results (1) diabetes, 8 weeks, NF-κB, PDGF-B and the expression of PKCα in the renal tissue was significantly increased (compared with normal control P