Effect of curcumin on cell cycle phase Tca8113 the impact of.docVIP

 PAGE \* MERGEFORMAT 8 Effect of curcumin on cell cycle phase Tca8113 the impact of [Abstract] Objective: To explore the effects of curcumin on Tca8113 (human tongue cancer cell line) cell lines induce differentiation and cell cycle phase effects. Methods: The MTT colorimetric assay and flow cytometry Tca8113 of curcumin on cell proliferation inhibition rate and changes in cell cycle and by electron microscopy to observe sub-cellular structures change. Results: The effect of curcumin on Tca8113 significantly inhibited cell proliferation in a dose-dependent. Flow cytometry, G 1 phase cells increased, S phase cells decreased, G 2. M-phase cells is relatively increased. Sub-cell structure: Cell cavitation, irregular nucleoli. Conclusion: Curcumin can inhibit cell proliferation Tca8113 to prevent the G 1 phase cells to S phase of the process. S phase cells decreased, G 2. M phase cells increased, the promotion of Tca8113 cell apoptosis. [Keywords:] curcumin; cell cycle; Tca8113 cell lines Curcumin (Curcumin) Turmeric is a traditional Chinese medicine extracted from the phenol pigment, with a wide range of pharmacological effects [1], such as anti-inflammatory, antioxidant, anticoagulant, blood fat, anti-atherosclerosis and anti-tumor and so on. Tca8113 target cells we used to explore the proliferation of curcumin on their cell cycle phase change, designed to provide a theoretical basis for the treatment of tongue cancer, it is to report on the results as follows: 1 Materials and methods 1.1 Material Tca8113 cell lines from Beijing Medical University Dental Hospital Loh gifts; curcumin were purchased in the Shanghai Chemical Reagent Procurement station (AR); RPMI1640 medium, MTT (tetrazolium Cypriot) purchased in the United States Sigma, Inc.; FBS were purchased in Changchun Institute of Biological Products. 1.2 Methods 1.2.1 MTT assay logarithmic growth phase of the Tca8113 cells. The 0.25% trypsin digestion, cells were collected, adjusting cell number o