Effect of glutamine on hepatic ischemia-reperfusion heat shock protein 70 gene expression.docVIP

 PAGE \* MERGEFORMAT 12 Effect of glutamine on hepatic ischemia-reperfusion heat shock protein 70 gene expression Of: Guo-Ping Liu, Feng Xiaodong, Zhu Wen River, Yang Guangshun, Zhou Wenping, Cheng Guangming [Abstract] Objective To study glutamine (Gln on hepatic ischemia-reperfusion heat shock protein 70 (HSP70 gene expression and its protective effects on the liver. Methods Male Wistar rats were 120, were randomly divided into 3 groups (n = 40: sham operation group (group 1, saline group (group 2 and glutamine groups (3 groups. preoperative 3 rats were injected intraperitoneally for 5 days Gln, 2 group were given normal saline .2,3 Group by Pringle’s method of hepatic artery occlusion, 35 minutes after the opening reflow, a group of anesthesia alone, laparotomy. were blocked before and 2,4,24 hours after reperfusion, blood and liver tissue were determined serum alanine aminotransferase (ALT, aspartate aminotransferase (AST, lactate dehydrogenase (LDH, tumor necrosis factor-@ (TNF @ liver tissue malondialdehyde (MDA content was detected by RT PCR the expression of liver HSP70mRNA. The results again 2,4,24 hours after reperfusion, group 3 MDA, ALT, AST, LDH and TNF @ level 2 group were significantly lower (P lt;0.05, and 3 HSP70mRNA liver tissues were significantly higher than group 2 and group 1 (P lt;0.05. Conclusions Gln gene can promote the expression of HSP70 in liver tissue, reduce hepatic ischemia reperfusion injury, inhibition of inflammatory cytokine release. [Keywords:] heat shock protein 70, glutamate, ischemia reperfusion, liver Abstract: Objective To investigate the influence of L glutamine on the expression of HSP70mRNA in liver and its protective role against ischemia / reperfusion damage.Methods One hundred and twenty male Wistar rats were divided randomly to three groups (n = 40): sham operation group (group 1), saline group (group 2) and glutamine group (group 3). Rats in group 2 were pretreated with 4ml 0.9% saline intraperitonall