Effect of dexamethasone on fetal rat palatal epithelial fusion of the ridge.docVIP

 PAGE \* MERGEFORMAT 12 Effect of dexamethasone on fetal rat palatal epithelial fusion of the ridge Study: An Feng Wu Jingfang Renjun Xu Wang Haoyu [Abstract] Objective: To study the dexamethasone on the fetal rat palatal epithelial ridge (Medial Edge Epithelium, MEE) cell apoptosis, proliferation and differentiation, to investigate the cause of dexamethasone in the pathogenesis of cleft palate deformities. Methods: acetic acid Dexamethasone induction of fetal cleft palate deformities, immunohistochemical method and image analysis of MEE cells bcl-2 and p53 genes at different times in the experimental group and control group expression. Results: In GD13.5 ~ 16.5 (GD, gestation day) MEE cells, the expression of bcl-2 and p53 to maintain the overall increasing trend; GD13.5 MEE cells, the expression of bcl-2 and p53 experimental group and control group, no significant difference; GD14.5 MEE cells, bcl- 2 and p5 expression significantly more than the control group the experimental group (P lt;0.01). Conclusion: Dexamethasone induced cleft palate MEE cells appeared reduced MEE cells by altering the bcl-2 and p53 gene expression and realization of MEE cells regulation of apoptosis and dexamethasone induced cleft palate is one possible mechanism. [Keywords:] bcl-2, p53; cleft palate; in the palatal epithelial ridge; cell apoptosis [ABSTRACT] Objective: To study the effectiveness and of dexamethasne (DEX) on the Apoptosis, differentiation and proliferation of mouse palatal medial edge epithelial (MEE) cells and its pathogenesis.Methods: The cleft palate of mice were obtained by injecting intraperitioneally with DEX acetate.The different periods expression of bcl-2 and p53 gene induced by DEX in embryonic palatine process tissues of experimental group and that of control group were detected by hybridization in situ.Results: In the experimental group of gestation day (GD) 13.5 to 16.5, the positive index of bcl-2 and p53 become higher; On GD 13.5 the signal