Effect of genistein on vascular smooth muscle cells of reactive oxygen species.docVIP

 PAGE \* MERGEFORMAT 12 Effect of genistein on vascular smooth muscle cells of reactive oxygen species OF: Like Jie Feng, Li Qin Shan, Liu Yang, Lu Hongling, Xiao Yu [Abstract] Objective: To investigate the effect of genistein on oxidized low density lipoprotein (oxidized low density lipoprotein, ox LDL in vascular smooth muscle cells induced by reactive oxygen species (reactive oxygen species, ROS generation. METHODS: The in vitro human umbilical vein smooth muscle cells (human umbilical vein smooth muscle cell, hUVSMC as object, 50 mg / L of ox LDL effector cells 2 h, and different concentrations of genistein (10, 30, 90 mol / L) on its intervention, the application flow cytometry and fluorescence spectrophotometry were used to detect total intracellular ROS levels. Results: hUVSMC stimulated by ox LDL 2 h, significantly increased intracellular ROS levels (P lt;0.01, genistein (10 mol / L reduced ox LDL-induced increase in intracellular ROS, genistein (30, 90 mol / L is to further improve the level of intracellular ROS. Conclusions: Genistein (10 mol / L to clear the intracellular ROS, and genistein (30, 90 mol / L increased intracellular ROS generation. [Keywords:] genistein, reactive oxygen species, oxidized low-density lipoprotein, vascular smooth muscle cells [Abstract] Objective: To investigate the effect of genistein on reactive oxygen species (ROS) production in vascular smooth muscle cell (VSMC) induced by oxidized low density lipoprotein (ox LDL). Methods: Human umbilical vein smooth muscle cells were pretreated with genistein in different concentrations (10, 30, 90 mol / L) and then treated by 50 mg / L of ox LDL for 2 hours. The levels of ROS were determined by using flowcytometry and fluorospectrophotometry. Results: The level of ROS in VSMC could be enhanced by ox LDL, genistein (10 mol / L) might decrease the level of ROS induced by ox LDL, but when the concentration of genistein arrived at 30 mol / L and 90 mol / L respectively, it