Effect of matrine on the growth of lung adenocarcinoma cells inhibit experimental study.docVIP

 PAGE \* MERGEFORMAT 8 Effect of matrine on the growth of lung adenocarcinoma cells inhibit experimental study Author: Geng Jun Jiang Jie DU Yoshinobu Yao talent side Chen Yang Chen Jun-Peng Zhang [Abstract] Objective: To study the differentiation inducing agent of matrine (Matrine) in vitro human lung adenocarcinoma SPC  A  1 cell growth inhibition effects. Methods: Different concentrations of matrine were dealing with lung adenocarcinoma SPC  A  1 cells, microscopic observation of the growth of cancer cells; determination of soft agar colony formation rate; MTT (thiazolyl blue) Determination of growth inhibition rate. Results: Different concentrations of matrine treatment of lung adenocarcinoma SPC  A  1 significantly inhibited cell growth, cell growth inhibition rate increased with the concentration of matrine increased. Conclusion: The Effect of matrine on lung adenocarcinoma SPC  A  1 cells significantly inhibited the growth. [Keywords:] lung adenocarcinoma SPC  A  1 cell growth inhibition by matrine Studies have shown that matrine has anti-inflammatory, anti-fibrosis, anti-virus and anti-tumor and many other biological activity [1]. To explore the matrine on the growth inhibition of lung adenocarcinoma cells, this study examined lung adenocarcinoma cells in vitro, through a certain concentration of matrine treatment of lung adenocarcinoma cells in a certain time, to observe the right lung adenocarcinoma cells growth inhibition effect, for clinical treatment of lung cancer to provide a theoretical basis. 1 Materials and methods 1.1 Material matrine purchased from Lianyungang, Jiangsu, preparation of 20g / L sterile solution of standby. Flow cytometry, for American products. Lung adenocarcinoma SPC  A  1 cell line by the College of Life Sciences, Xiamen University to provide. Fetal calf serum were purchased from Hangzhou Sijiqing bio-engineering company, selected experiments logarithmic phase cells. 1.2 cell culture were rando