Effect of melatonin on the calyx calyculin-induced hyperphosphorylation of tau protein in the impact of.docVIP

 PAGE \* MERGEFORMAT 7 Effect of melatonin on the calyx calyculin-induced hyperphosphorylation of tau protein in the impact of [Abstract] Objective To investigate the melatonin (Mel) on the calyx calyculin (CA) in the tumor cells into neurons induced hyperphosphorylation of tau protein and its mechanism. Methods of wild-type mouse tumor cells into neurons (N2awt), given to CA treatment, or while giving 50μ mol / L Mel treatment, by immunofluorescence detection of the level of tau protein phosphorylation, 32P  specific substrate labeling detection of GSK  3 activity, immune blot detection of P  Ser9  GSK  3β and GSK  3β expression levels to calculate P  Ser9  GSK  3β /GSK  3β ratio. Results ① CA can be N2awt cells to sites of tau protein in Ser396/404 and Ser198/202 sites of hyperphosphorylation, accompanied by increased activity of GSK  3 and P  Ser9  GSK  3β expression levels lowered. ② Mel pairs of CA induced hyperphosphorylation of tau protein has a protective effect; Mel at the same time against CA-induced GSK  3 activity increased and P  Ser9  GSK  3β expression levels lowered. Conclusion Mel reduce the CA induced hyperphosphorylation of tau protein and its mechanism may be related to regulation of intracellular P  Ser9  GSK  3β expression levels and changes in GSK  3 activity. [Keywords:] calyx calyculin; tau; melatonin; hyperphosphorylation; Alzheimer’s disease Effect of melatonin on calyculin A  induced tau hyperphosphorylation ABSTRACT: Objective To investigate the in vivo effect of melatonin (Mel) on calyculin A (CA)  induced tau hyperphosphorylation in neuroblastoma cells (N2awt). Methods We treated N2awt cells with CA or CA and 50μ mol / L Mel, detected the level of tau phosphorylation with immunofluorescence, and assayed the activities of GSK  3 and the ratio of GSK  3β phosphorylated at Ser9 site to total GSK  3β . Results CA treatment led to tau hyperphosphorylation accompanied wi