Effect of melatonin on spinal cord injury in iNOS expression after.docVIP

 PAGE \* MERGEFORMAT 14 Effect of melatonin on spinal cord injury in iNOS expression after Study: Herman Li Dong Wu Yonggang Zhao Lijun Yang Xiuling paragraph [Abstract] Objective: To investigate the melatonin after spinal cord injury in rats induced nitric oxide synthase (iNOS) expression. Methods: Preparation of Modified Allen’s impact spinal cord injury model, adult SD rats 110 randomly divided into sham injury, injury and drug treatment group, 3 groups, which injury group and medication group 50, sub-5 time points (8 hours, 24 hours, 72 hours, 7 days, 14 days were sacrificed, the false injury group, 10 rats were killed 14 days after surgery, using HE staining and immunohistochemistry of spinal cord tissue after spinal cord injury in iNOS protein expression. Results: Compared with the injury, drug treatment group, iNOS expression in spinal cord tissue after injury significantly decreased significantly (P lt;0.05). CONCLUSION: Melatonin can inhibit the expression of iNOS protein on the protective effect of spinal cord injury. [Keywords:] spinal cord injury; melatonin; inducible nitric oxide synthase Abstract Objective: To investigate the influence of Melatonin on the induction of inducible nitric oxide synthase (iNOS) expression in spinal cord upon spinal cord injury (SCI) in rats. Methods: SCI model was reproduced according to Allen’s method with modification. One hundred and ten adult SD rats were randomly divided into three groups: sham injury group (n = 10), the spinal cord injured group (n = 50), and SCI with melatonin treatment group (n = 50). For the SCI groups with or without melatonin treatment, rats were killed at different time (8h, 24h, 72h, 7d, 14d). And rats of sham injury group were killed at 14d post SCI operation. HE staining and Immunohistochemistry method were employed to detect iNOS expression in spinal cord. Results: Melatonin treatment significantly reduces the induction of iNOS expression in spinal cord of rats subjected t