Effects of metformin on lipid toxicity in islet cell function.docVIP

 PAGE \* MERGEFORMAT 18 Effects of metformin on lipid toxicity in islet cell function Of: Wang Yuhuan, at Ting, Yan Lili, Zheng Huadong, Wu Juanjuan, Zhang Jing [Abstract] Objective metformin (MF) in the free fatty acid (FFA)-induced apoptosis in pancreatic islet cells and its mechanism. Methods tetrazolium blue (MTT) colorimetric FFA observed cell proliferation in mice Tc3 inhibition; flow cytometry of apoptosis; immunocytochemistry and Western blot, p JNK, pc jun in the FFA, specific JNK inhibitor (SP600125) blocked JNK signaling pathway and the MF case The expression under. Results of in vitro growth Tc3 FFA significantly inhibited cell and induce apoptosis; MF can significantly reduce the process of apoptosis. immunocytochemistry and Western blot showed that, FFA induced Tc3 apoptosis associated with p JNK and the increase of pc jun; Tc3 cells pretreated with SP600125, can significantly reduce the p JNK and pc jun activation, while the MF is not able to inhibit FFA induced p JNK activation. Conclusions MF FFA can inhibit mouse Tc3 mediated apoptosis, but not by JNK signaling pathway play a role. [Keywords:] free fatty acid; Tc3 cells; JNK signaling pathway; metformin; lipid apoptosis ABSTRACT: Objective To investigate the effects of metformin on apoptosis in mouse Tc3 cells induced by free fatty acids (FFA) and the underlying mechanism. Methods MTT assay was used to observe the inhibitory actions of FFA on the proliferation of Tc3 cells. The apoptosis rate of the cells was detected by flow cytometry. The expressions of p JNK and pc jun were detected by immunocytochemistry and Western blot. Results The proliferation of Tc3 cells was remarkably inhibited by FFA in vitro, and significant apoptosis was present. Metformin could inhibit the apoptosis significantly. Immunocytochemistry and Western blot results showed FFA induced activation of p JNK, pc jun. As a JNK inhibitor, SP600125 partly suppressed FFA induced activation of p JNK and pc jun; ho