Efficient pseudotyped retroviral vector-mediated gene transfer in rabbit smooth muscle cells.docVIP

 PAGE \* MERGEFORMAT 12 Efficient pseudotyped retroviral vector-mediated gene transfer in rabbit smooth muscle cells Of: Pei Fei, He Rui, Lijun Yan, Yu Hong [Abstract] Objective To construct the retroviral vector pseudotyped MuLV / VSV G, and for transfection of rabbit smooth muscle cells, smooth muscle cells of rabbit looking for a highly efficient gene transfer vector. Methods lacZ reporter gene construct containing the pseudotyped retrovirus vector MuLV / VSV G, titer determination, and transfection of rabbit smooth muscle cells, transduction efficiency was observed. and with the MuLV transduction efficiency compared. was constructed in the MuLV / VSV G vector, the virus titer was 6 ~ 7.8 106CFU, transfection efficiency of rabbit smooth muscle cells (92 + -12)%. and MuLV transduction efficiency (24 + -5)%. Conclusion The successful construction of the retroviral vector pseudotyped MuLV / VSV G vector, the vector as an efficient vector for gene transfection of rabbit smooth muscle cells. [Keywords:] pseudotyped retroviral vector, smooth muscle cells, transfection ABSTRACT: Objective To construct pseudotyped retroviral vector MuLV / VSV G and transfer it into rabbit smooth muscle cells (SMC) in order to provide a high efficiency vector for SMC gene transfer. Methods We constructed pseudotyped retroviral vector MuLV / VSV G containing the previously reported gene lacZ, determined the titer, and determined the efficiency of gene transfer into SMC mediated by pseudotyped retroviral vector MuLV / VSV G. Finally the transfer efficiency was compared with that by MuLV. Results MuLV / VSV G vector was constructed. The titer of the vector was 6-7.8 106CFU, the transfer efficiency was (92 + -12)% by using MuLV / VSV G vector and (24 + -5)% by MuLV vector. Conclusion Pseudotyped retroviral vector MuLV / VSV G which was constructed successfully is a kind of high efficiency gene transfer vector in smooth muscle cells. KEY WORDS: pseudotyped retroviral vect