Endostatin combined with radiation on human lung adenocarcinoma A549 cells.docVIP

 PAGE \* MERGEFORMAT 17 Endostatin combined with radiation on human lung adenocarcinoma A549 cells [Abstract] Objective To study the irradiation combined with endostatin on lung adenocarcinoma cell line A549 and the implications for vascular endothelial growth factor (VEGF) expression. Methods different concentrations of endostatin and A549 cells at different time, MTT method selective growth inhibition rate of 20% of the drug concentration (IC20); cells were divided into control group, the irradiation group, simple drug group, drug and radiation combined group, the joint component 3 subgroups were cultured 24h dosing , 48h and 72h after irradiation 2Gy. Comparison of survival fraction, select the best time for drugs; with 200mg / L drug concentration in the role of the experimental group, after 48h drug 0,2,4,6,8 Gy dose of X-irradiation, Draw cell survival curve, compared with the irradiation group, compared with calculated sensitization (SER); ELISA method to detect the VEGF values in supernatants. Results 200mg / L concentration of endostatin radiosensitization after 48h in drug The most obvious sensitization ratio 1.61,1.04; irradiation combined with endostatin can reduce lung cancer after radiation therapy the level of expression of VEGF. Conclusion endostatin on in vitro non-small cell lung cancer cell line A549 has a radiosensitizing effect, and most good exposure time for the drug after 48h, the possible mechanisms to increase the radiosensitivity of tumor cells to reduce the levels of VEGF. [Keywords:] cancer; non-small cell lung; cell line; tumor; endostatin; radiosensitization ABSTRACT: Objective To study the synergistic effect of endostatin (YH 16) and irradiation on human non small cell lung cancer (NSCLC) cell line A549 and the expression level of vascular endothefial growth factor. Methods A549 cells were exposed to various concentrations of endostatin for different time. The optimal concentration giving 20% inhibition concentrat