Ethaselen mice transplanted liver cancer (H22) and anti-tumor effect of Lewis lung cancer.docVIP

 PAGE \* MERGEFORMAT 12 Ethaselen mice transplanted liver cancer (H22) and anti-tumor effect of Lewis lung cancer [Abstract] Objective To observe Ethaselen mice transplanted liver cancer (H22) and anti-tumor effect of Lewis lung cancer and to explore its possible anti-tumor mechanism. Methods mice transplanted liver cancer (H22) and Lewis lung cancer model, each is 60, respectively, to 5 groups: negative control, positive control (CTX) and Ethaselen 3 dose groups, 12 in each group. inoculated tumor in 2 days to 8 days 5 groups were injected intraperitoneally 5g / L sodium carboxymethyl cellulose, 60mg/kg CTX and 25,12.5,6.3 mg / kg Ethaselen. discontinuation after 3d (11 days) in each treatment half of the animals, calculated suppression tumor rate, and observed by flow cytometry on the cell cycle, the other half animal life extension rate observed. Results Ethaselen in 25,12.5,6.3 mg / kg dose in mice transplanted liver cancer H22 tumor suppression tumors were 52.5%, 43.6% and 30.2%, life extension rates were 56.6,38.7% and 14.2% inhibition of mouse Lewis lung cancer rates were 43.8%, 29.6% and 18.9%, respectively to extend the life 47.3%, 17.9% and 6.3%. Ethaselen each dose group was significantly higher in tumor cell apoptosis (P lt;0.05), and 25.0mg/kg group showed S phase arrest (P lt;0.05). Conclusion Ethaselen mice transplanted liver cancer (H22) and Lewis lung cancer with anti-tumor effect and increase with the dose significantly enhanced inhibition of tumor. Its anti-tumor effect may be through the induction of apoptosis, also interfere with tumor cell cycle, leading to S phase arrest achieved. [Keywords:] Ethaselen, H22 hepatoma lines, Lewis lung cancer lines, flow cytometry ABSTRACT: Objective To observe the anti tumor effect of ethaselen on transplanted liver cancer (H22) and Lewis lung cancer mice. Methods The transplanted liver cancer (H22) and Lewis lung cancer mice models were established. Each genus consisted of 60 mice, which were div