Evening Primrose Oil capsules compound content of linolenic acid in the determination of γanalysis.docVIP

 PAGE \* MERGEFORMAT 5 Evening Primrose Oil capsules compound content of linolenic acid in the determination of γanalysis Author: Zhao Wei, Cai Guang Zhi, Wang Shasha, Xiao-Yan Xie, Gong Ji-yu [Keywords:] evening primrose oil; γ  linolenic acid; determination; GC  MS; gas chromatograph Fund Project: Jilin Provincial Science and Technology Agency major projects funded scientific and technological development issues Evening primrose oil (evening primrose oil) collected from the perennial herb Onagraceae Evening primrose (Oenothera biennis L) seeds, containing a variety of fatty acids and essential fatty acids are mostly human, the main active ingredient of γ  linolenic acid (γ  linolenic acid, the chemical structure of cis-3  6,9,12  18 carbon acid), in the body can be transformed into arachidonic acid for prostaglandin and the composition of biofilm provides precursors. Over the years, scholars at home and abroad evening primrose oil to a large number of studies now confirm that a significant anti-inflammatory, anti-oxidation, anti-thrombosis, lower blood sugar, blood fat, anti-platelet aggregation and other pharmacological effects, clinical mainly used in diabetes mellitus, hyperlipidemia, atherosclerosis, coronary heart disease, chronic renal insufficiency and other diseases has been widely used in medical, health and food industry and other fields. In this paper, gas chromatography evening primrose capsules γ  compound content of linolenic acid, by GC-MS methods used in analysis of other essential fatty acids. An apparatus and materials Electric constant temperature water bath, rotary evaporator, Shimadzu GC  14C gas chromatograph. Urea, KOH, methanol, ethanol, hydrochloric acid, anhydrous sodium sulfate, were of analytical grade. γ  acid methyl ester reference substance Flax (China Pharmaceutical and Biological Products Institution, Lot: 898  200101). 2 Methods and Results 2.1 urea inclusion [1] will be refined evening