Estrogen receptorgene promoter methylation and its protein expression.docVIP

 PAGE \* MERGEFORMAT 16 Estrogen receptorgene promoter methylation and its protein expression Of: BRIDGE King, Sushi Zhen, Li Hongzhi, Lin Beibei, white clover [Abstract] Objective: To detect the estrogen receptor @ (estrogen receptor @, ER @) gene promoter CpG island methylation in breast cancer ER @ promoter methylation and its protein expression and clinical pathology in . Methods: Immunohistochemical detection of two-step EnVisionTM 20 cases of normal breast tissue, 44 cases of breast cancer ER @ gene expression, while the use of methylation specific PCR (Methylation-Specific PCR, MSP) and sequencing were used to detect ER @ gene promoter A, and B, the methylation status of CpG islands. Results: 32 patients with ER @-positive breast cancer, ER @ A, promoter, B methylation rates were 28.2% and 18.8% negative breast cancer patients with ER @ .12 organizations, ER @ A, promoter, B methylation rates were 66.7% and 58.3%. ER @-positive breast cancer group, ER @ negative breast cancer group, breast cancer group ER @ promoter A, B methylation rate was significantly higher than normal (P lt;0.05), ER @ ER @ negative breast cancer promoter group A, B methylation rate than ER @-positive breast cancer group were significantly increased (P lt;0.05). Breast Cancer ER @ expression and promoter methylation was a significant negative correlation between. Conclusion: The breast cancer gene silencing ER @ A, and its promoter, B District, a CpG island of relation, which may be of poor prognosis of breast cancer molecular detection indicators. [Keywords:] breast cancer, estrogen receptor, DNA methylation, CpG island, methylation-specific PCR Abstract: Objective: To detect the methylation of CpG island in estrogen receptor alpha (ER @) gene promotor in breast cancer, and study the relationship among ER @ gene promotor methylation, ER @ expression, and the clinicopathological characteristics in breast cancer. Methods: The EnVisionTM two-step immunohistochemistry meth