Expression of bifunctional antibodies and prediction.docVIP

 PAGE \* MERGEFORMAT 7 Expression of bifunctional antibodies and prediction Keywords:: single-chain antibody Single-chain antibody addition to its own therapeutic role, but also as a carrier combined with cytokines such as building a dual function as the antibody used in cancer treatment guide. It is reported in the literature [1 ~ 3] The full-derived factor-2 gene fusion into a single protein detected by anti-tumor activity of the fusion protein has a dual activity, but the affinity of the fusion protein and anti-tumor activity of the respective functions of the more-derived factor and activity change could be due to structural changes in fusion proteins lead to functional and activity changes. The use of network resources, bioinformatics analysis of the secondary structure of the fusion protein and its physical and chemical properties, in order to further explore the single-chain bifunctional antibody fusion protein gene to provide a basis. 1 pairs of functional antibody research With the development of molecular biology, tumor susceptibility gene therapy to become a hot research scholars from various countries [3 ~ 5]. The target gene into the target cells for gene therapy and an important part of the process, because the purpose of the efficiency of gene transfer into target cells will directly affect the level of the effect of gene therapy, and even the success or failure. By retrovirus-mediated gene transfer is the biggest problem faced by the less efficient viral transduction, one of the reasons is due to stability of packaged difficult to obtain a higher titer of infectious retrovirus packaging cell line. Single-chain antibody (ScFv) antibodies derived from the Fv, the antibody heavy chain variable region and light chain variable region connected by a linker formed, ScFv antibody with a natural affinity, but only a complete antibody molecule of the six sub - one. With the molecules are small, penetrating power and easy ac