Fluoxetine in the regulation of fetal rat neural stem cells in the Notch1 pathway gene expression changes.docVIP

 PAGE \* MERGEFORMAT 19 Fluoxetine in the regulation of fetal rat neural stem cells in the Notch1 pathway gene expression changes Of: Suiyu Xiu, Zhang Jun, Guo Yijing, Yi Sun [Abstract] Objective: To investigate the Notch1 signaling system and fluoxetine in promoting neural stem cells (NSCs) proliferation. Methods: Different concentrations of fluoxetine interfere with NSCs, to obtain optimum levels. Notch1 signaling pathway inhibitors were given and fluoride DAPT Meperidine NSCs intervention, the proliferation of NSCs by MTT testing, real time PCR signals were detected Notch1 gene expression of each factor. Results: (1) different concentrations of fluoxetine interfere with cell 48 h, 10,15 and 20 mol L-1 group than the activity of NSCs 0 mol L-1 group, the difference was statistically significant (P lt;0.01); (2) compared with the control group, fluoxetine group of cell activity increased, DAPT group activity lower, the difference was statistically significant (P lt;0.001). and the intervention group compared with fluoxetine, fluoxetine plus DAPT group cell activity decreased significantly (P lt;0.001); (3) and the control group compared to, DAPT group Hes1mRNA and Hes5 mRNA expression was significantly decreased (P lt;0.001), fluoxetine group Notch1 mRNA, Hes1 mRNA and Hes5 mRNA expression was significantly higher (P lt;0.001 or P lt;0.01); intervention group compared with fluoxetine ratio, fluoxetine plus DAPT group Notch1 mRNA, Hes1 mRNA and Hes5 mRNA expression were significantly decreased (P lt;0.001). Conclusion: Fluoxetine may signal transduction by regulating Notch1 promote proliferation of NSCs. [Keywords:] neural stem cells; fluoxetine; Notch1 signaling system; rat [Abstract] Objective: To investigate whether the effect of fluoxetine on cell proliferation involves Notch1 signaling in cultured neural stem cells (NSCs). Methods: MTT assay was used to evaluate cell viability. The expression of Notch1 signaling components (including Notch1 m