Fluticasone propionate in asthmatic airway remodeling and the expression of TGFβ 1.docVIP

 PAGE \* MERGEFORMAT 5 Fluticasone propionate in asthmatic airway remodeling and the expression of TGFβ 1 [Keywords:] fluticasone propionate; asthma; airway remodeling; transforming growth factor-β ; mice In recent years, growing body of research evidence that exists apart from bronchial asthma airway inflammation and nonspecific airway hyper-reactivity, but also the existence of airway remodeling, histology showed epithelial cell hyperplasia, subepithelial collagen deposition and fibrosis, as well as the hypertrophy of smooth muscle cell proliferation heterogeneity of change caused by the increase in airway wall thickness, leading to irreversible airflow obstruction and airway hyper-responsiveness of the sustained [1]. is generally agreed that airway remodeling is caused by inflammation after tissue injury is not completely or excessive repair due to [2]. glucocorticoid control asthma is currently the most effective anti-inflammatory drugs, but the role of airway remodeling-related research is currently reported less [3-4]. In this study, asthmatic mice Gas airway remodeling model, observe the early application of fluticasone propionate, the wall thickness of airway wall, smooth muscle thickness and changes in the expression of TGF  β 1, and further explore its effects on airway remodeling and the possible mechanism of action. 1 Materials and methods 1.1 Materials Balb / c mice were 30, male, body weight (20 ± 2) g, Fourth Military Medical University Experimental Animal Center. Chicken egg albumin (ovalbumin, OVA), fluticasone propionate powder (Sigma, USA); TGF  β 1 rabbit anti-mouse polyclonal antibody (Beijing Zhongshan Biological Engineering Co., Ltd.); streptavidin-avidin  biotin  peroxidase complex (strept avidin  biotion complex, SABC) immunohistochemical staining kit (Beijing Zhongshan Biological Technology Co., Ltd.). 1.2 Methods 1.2.1 Animal grouping and model 30 male mice were randomly divided into the asthma group (A group), norm