Glutathione in rats with ventilator-associated lung injury.docVIP

 PAGE \* MERGEFORMAT 16 Glutathione in rats with ventilator-associated lung injury [Abstract] Objective To study the glutathione (GSH) on rat ventilator-associated lung injury (VILI) in rats. Methods 24 healthy male SD rats were randomly divided into 3 groups (each n = 8) , A group for the spontaneous breathing group; B group, tidal volume mechanical ventilation group; C group, tidal volume mechanical ventilation plus GSH treatment. B, C 2 group of respiratory parameters: tidal volume (VT) = 30 ml / kg, frequency ( P) is 40 times / min, ventilation time was 4 h. Line 1 hour arterial blood gas analysis, calculation of oxygenation index (PaO2/FiO2). experiment is completed to collect specimens of lung tissue and lung lavage. light microscopy of lung tissue pathological changes and immunohistochemical detection of Fas / FasL protein, lung wet / dry weight ratio (W / D), lung tissue malondialdehyde (MDA), superoxide dismutase (SOD), lung lavage fluid leukocyte count, tumor necrosis factor (TNF-@) and total protein. Results and A group, B group significant pathological changes in lung tissue, Fas / FasL protein increased, W / D, white blood cell count (WBC), TNF-@, MDA and other indicators increased, while SOD and ventilation PaO2/FiO2 decreased after 3 h; and the B group, C group significantly reduced pathological changes in lung tissue, Fas / FasL protein decreased, W / D, WBC, TNF-@, MDA and other indicators decreased, SOD, PaO2/FiO2 increased. Conclusion GSH of VILI have a protective effect. [Keywords:] ventilator-associated lung injury; mechanical ventilation; glutathione Abstract: Objective To investigate the protective effects of reduced glutathione on ventilator-induced lung injury (VILI) in rats.Methods 24 male Sprague-Dawley rats were randomly divided into 3 groups (n = 8 each): group A received room air without mechanical ventilation; group B received mechanical ventilation of large tidal volume (VT) with room air; group C was treated with mechan