Glutathione S transferase in the gastrointestinal cancer and breast cancer clinical significance of expression.docVIP

 PAGE \* MERGEFORMAT 8 Glutathione S transferase in the gastrointestinal cancer and breast cancer clinical significance of expression Of: Bo Aihua Li Haifeng Zhang Xiaoli Wang Xiaoyin Keywords: placental glutathione S transferase; gastric adenocarcinoma; colorectal adenocarcinoma; breast cancer; immunohistochemistry Glutathione S-transferase (GST) has five subtypes, including placental GST (GST) accounted for about 90% higher expression in epithelial tumors, and GST high expression is closely related to tumorigenesis and drug resistance 1 3, so GST is known as tumor markers or drug resistance genes. Currently on the GST expression in epithelial tumor-reported, this paper SP immunohistochemical technique to detect gastrointestinal cancer and breast cancer GST expression, overexpression of the GST the biological characteristics of cancer cells and lead to tumor drug resistance mechanisms, designed to provide the basis for clinical treatment. 1 Subjects and methods 1.1 Case information 2004 to 2007, surgical and hospital tumor resection Fifth Hospital of Zhangjiakou City, gastric adenocarcinoma 64 cases, of which 40 cases of male and 24 female patients aged 30 to 79 (mean 53.5 21.4) years; 66 cases of colorectal cancer, male 49 patients, 17 females, aged from 32 to 84 (mean 58 16.7) years, mean age 58 years; 84 cases of breast cancer, all female, age 27 to 71 (mean 49 21.8) years. 1.2 Experimental Methods Section 1.2.1 Production Fresh specimens were fixed in 10% neutral formalin overnight, the conventional gradient ethanol dehydration, xylene, and embedded in paraffin, serial sections of thickness 5 m. Part of the routine HE staining sections for case diagnosis. Other sections at 37 storage boxes waiting to be dyed. 1.2.2 Immunohistochemistry Section GST rabbit anti-human antibody serum, produced by the United States MAXIM; streptomycin resistant organism protein peroxidase method (SP) immunohistochemistry kit and diamino