Habitual abortion on apoptosis and trophoblast Fas FasL expression.docVIP

 PAGE \* MERGEFORMAT 9 Habitual abortion on apoptosis and trophoblast Fas FasL expression [Keywords:] habitual abortion trophoblast apoptosis in Fas, FasL Habitual abortion is a continuous occurrence of 2 or more than 2 spontaneous abortions, the rate of about 1% [1]. Modern reproductive immunology research shows that the cause of habitual abortion except chromosomes, anatomy and endocrine abnormalities, but about 50% 60% immunity. has now been confirmed, apoptosis (apoptosis) is a physiological phenomenon, but the cells are stagnant or too fast can cause disease. by the Fas / FasL-mediated activation of T lymphocytes specific apoptosis in peripheral T lymphocytes play an important role in immune tolerance [2]. trophoblasts of placental apoptosis and Fas, FasL protein expression, the cellular and molecular level to clarify habitual abortion pathological changes and immune pathogenesis, provide a theoretical basis for clinical treatment. Subjects and methods 1. Object: 2004 to 2007 in our hospital of 28 patients with habitual abortion, of which 18 cases of missed abortion, inevitable abortion in 6 cases, 4 cases of incomplete abortion. Patients were early abortion (gestational age ≤ 12 weeks), age 23 to 38 years old. abortion diagnostic criteria based on referenced literature [3]. Select the same period in our hospital family planning clinics receiving the health of women in early pregnancy abortion in 25 cases, no previous history of abnormal pregnancy, normal delivery have a history of more than child, aged 24 to 35 years of age for the normal control group. 2. Specimen processing: a product from the uterine scrapings of pregnancy, the placenta of the villi or in part, rinsed with saline to remove blood, immediately fixed in 10% neutral formalin 24 h, embedded in paraffin, serial sections. Each organization slice thickness of 5 μm, to 60 ℃ oven for 72 h, the slices close adhesion. 3. Immunohistochemistry: Fas and FasL protein chain by immuno