HE ethanol extract of sciatic nerve injury in immune function of mice.docVIP

 PAGE \* MERGEFORMAT 9 HE ethanol extract of sciatic nerve injury in immune function of mice Of: Hui Cao Jian Liu Biao Yin Weitian [Abstract] Objective To study the ethanol extract hematoxylin (SME sciatic nerve injury on the immune function of mice. Methods adult male Balb / c mice 60, were randomly divided into the SME low dose group and high school control group, the right line sciatic nerve transection and anastomosis, 3,5,7,14,28 d respectively after determination of lymphocyte proliferation by MTT conversion capacity, ELISA method for measuring serum circulating immune complexes (CIC concentrations. Results of mouse lymphocytes at all time points proliferation and transformation capacity and serum CIC concentration and SME clear dose effect relationship. Conclusion SME sciatic nerve injury in mice can affect the cellular and humoral immune function, and with a clear dose effect relationship exists. [Keywords:] HE ethanol extract, nerve damage, immune In the past, Chinese herbs for promoting blood circulation to the immune activity screening, the public surplus hematoxylin shows the effectiveness of the immune suppression (1), confirmed by hematoxylin has been reported in rat ethanol extract significantly inhibited the activity of lymphocytes (2) . many related studies have shown that after peripheral nerve injury, damage occurred there from the immune response to the inhibition of nerve regeneration, the stronger immune response, nerve fiber regeneration and functional recovery worse, inhibit the immune response triggered by nerve injury nerve regeneration play a catalytic role (3). This paper on in vitro activity of immune cells and serum circulating immune complexes (CIC determination of ethanol extract of hematoxylin sciatic nerve injury in Balb / c mice immune function, in order to apply for the surrounding hematoxylin theoretical basis for nerve regeneration to find. 1 Materials and methods 1.1 Animals and reagents Male Balb / c