Heavy metal exposure can alter the accumulation of fat in C. elegans.docVIP

 PAGE \* MERGEFORMAT 20 Heavy metal exposure can alter the accumulation of fat in C. elegans [Abstract] Objective: To study the heavy metal exposure on C. elegans fat storage effects of acute poisoning, identification of heavy metal exposure occurs in C. elegans expression pattern changes associated with fatty acid metabolism genes. Methods: silver, cadmium, chromium, copper, mercury, manganese and As a test of zinc metal, Nile Red fluorescent probe staining of intestinal nematodes exposed to heavy metal content of fatty acids and determination of fatty acid metabolism in C. elegans exposure to heavy metals related to the level of gene transcription. Results: In the analysis of heavy metals in high concentrations of silver, chrome and copper exposure can significantly induce the accumulation of fat increases intestinal nematodes, and the high concentration of cadmium exposure can significantly reduce the nematode intestinal fat accumulation. Moreover, compared with the control group, high concentrations of silver and chromium exposure is not obvious influence gene pod 2 , gei 7, lbp 8, gpd 3, W05G11.6, C03H5.4 C07E3.9 and the expression levels of nematodes in the exposed animals, gene fat 5, fat 6 and fat 7 expression was significantly increased, lbp 1, acs 2 and ech 1 expression was significantly reduced. Conclusion: High concentrations of silver, chromium, copper and cadmium exposure can lead to abnormal accumulation of fat in C. elegans intestinal changes, including exposure to high concentrations of silver and chrome fat accumulation led to an increase nematode The main reason may be attributed to exposure to saturated fatty acids in vivo mitochondrial oxidative metabolism and fatty acid binding exception. [Keywords:] fat accumulation; metal exposure; metabolism; Nile Red staining; C. elegans Bioassays, usually used in monitoring scheme in toxicological studies, can be explored to monitor the acute toxicity of aquatic effluents according to the gu