High-yield clavulanic acid-resistant mutant strain selection glycerol and physiological characteristics of.docVIP

 PAGE \* MERGEFORMAT 19 High-yield clavulanic acid-resistant mutant strain selection glycerol and physiological characteristics of Authors: Chenxi Yong Shi Yufang Wang Guo-Fen Pu Yang Meifen [Abstract] so as to bring a small stick of Streptomyces XC-1-35 as the starting strain by ultraviolet radiation 60s, combined with glycerin tolerance to strains of rational selection, mutation breeding to be a better strain XC-5-48, titer up to 1970μ g/ml. The slope of the strain continuously passaged four times more potency and stability in the 1800μ g/ml. Physical characteristics to the strains studies have shown that a strong dynamic of its glycerol-specific induction of glycerol transport system (GTS). With XC-5-48 strain as the test strain, when the initial concentration of glycerol medium 1.5%, and 60h fermentation of additional 1.0% when the glycerol fermentation titer of up to 2668μ g/ml, higher than the starting strain (318μ g/ml) increased 7.4-fold. The process applied to a total of 10 batches of 15L fermentor fermentation tests, 96h clavulanic acid, when the average titer 2103μ g/ml. [Key Words] Clavulanic acid; biosynthesis; glycerol; strain breeding; strain characteristics of ABSTRACT The clavulanic acid-producing strain Streptomyces clavuligerus XC-1-35 with the yield of 318 μ g / ml was irradiated by UV for 60 s, and a glycerol-tolerant mutant XC-5-48 with a high productivity of 1,970 μ g / ml was screened out. Physiological characteristics for strain XC-5-48 showed that this mutant not only had a stable productivity of clavulanic acid after subculturing for fourth generations, but also had active glycerol transport system (GTS) which was specifically induced by glycerol. Under the optimum concentration of 1.5% glycerol in fermentation medium and feeding of 1% glycerol as precursor after 60 h of incubation, the titer of 2,668 μ g / ml was obtained in shake flask culture, an increase of 7.4 times compared with the original strain. Fermentation for 96 h in