Hirudo nipponica in vitro anticoagulant activity in different extract parts of Experimental Research.docVIP

 PAGE \* MERGEFORMAT 6 Hirudo nipponica in vitro anticoagulant activity in different extract parts of Experimental Research Author: YAO Nan YU Shun-Hui Yu You-Bin-phase [Abstract] Objective To study the Japanese medical leech extract in different parts of the anticoagulant activity, for the Japanese medical leech to provide a basis for new drug development. Methods SD rats were the main abdominal vein blood, measured in vitro application of coagulometer prothrombin time (PT), thrombin time (TT) and activated partial thromboplastin time (APTT). The results of the total Japanese medical leech extract in rats can PT, TT, APTT time was significantly extended; petroleum ether parts can rat PT, APTT extension. Conclusion Hirudo traditional Chinese medicine in Japan’s low-polar small molecules with anticoagulant activity. [Keywords:] Japanese medical leech anticoagulant As a medicinal leech, a long history. In China, the leech only contained in the ‘Shen Nong Ben Cao Jing’, which claims to ‘by bad blood,’’Po Xue Zheng Jia accumulation of blood stasis’. Is widely used in clinical thrombosis, coronary heart disease and cerebral edema [1]. There are hundreds of the world’s species of leeches, China has nearly one hundred kinds. ‘The 2005 edition of Chinese Pharmacopoeia containing relevant leech species can leech Whitmania pigra Whitman, Japanese medical leech Hirudo nipponica Whitman and willow leech Whitmania acranulata Whitman 3 Zhong [2]. Is now recognized as the active ingredient leech hirudin, which is a career in medicine extracted from the leech of the salivary glands of a polypeptide, is the role of a strong thrombin inhibitor with anticoagulant, anti-thrombotic and other pharmacological effects. The application of traditional Chinese medicine traditional medicine concocted by the leech, the leech has to go through water, or sand being burnt to death dried fried, hirudin completely destroyed [3,4], but Leech Poxue, pass through the effect is still preserved,