Homocysteine in patients with peripheral blood mononuclear cells of breast cancer tissue factor expression.docVIP

 PAGE \* MERGEFORMAT 12 Homocysteine in patients with peripheral blood mononuclear cells of breast cancer tissue factor expression Of: Wangning Ju, Chen Yaoping, Yang Baozhen [Abstract] Objective To investigate whether Hcy induced peripheral blood mononuclear cells in patients with breast cancer (Monocyte, Mn) expression of tissue factor (TF). Methods with different concentrations of Hcy [12.5mol / L ( group), 25mol / L (III group) , 100mol / L ( group), 50mol / L (V group)] Mn breast cancer patients on short-term culture, the crushed cells, ELISA was used to detect the TF expression. Results With increasing concentrations of Hcy, TF in breast peripheral blood mononuclear cells in patients with carcinoma of the expression level gradually increased in a dose dependent manner. which and III, , V group was significantly (P all lt;0.01), group , V group comparison, III group and group , III and V group differences between groups were statistically significant, and group group, group and III group, group and V group was no significant difference. Conclusion Hcy by Mn breast cancer patients induced the expression of TF, resulting in invasion and metastasis of breast cancer play a role. [Keywords:] homocysteine, monocytes, tissue factor, breast cancer Abstract: Objective To explore the effects of homocysteine #8203;#8203;on expression of tissue factor (TF) in peripheral monocyte in cases with breast carcinoma.Methods T The peripheral monocyte were incubated with the culturing liquid of different Hcy concentrations for short time, and then smashed.The levels of TF was detected using ELISA method.Results As incubated with Hcy, monocytic cells can be induced to express TF, which is upregulated in dose-dependent pattern.Among the groups, there is statistical difference between group and group III, group and group , group and group V, group and group , group and group V, group III and group V. Conclusion Hcy induce Monocytic cells to express TF and p