Honeysuckle 3 ingredients in the ultra-high speed liquid chromatography.docVIP

 PAGE \* MERGEFORMAT 8 Honeysuckle 3 ingredients in the ultra-high speed liquid chromatography [Abstract] Objective To establish a high-speed liquid chromatography (UFLC) Simultaneous determination of luteolin in honeysuckle, hyperin and chlorogenic acid. Methods UFLC law column for the XR-ODS column Shimadzu (75 mm × 3.0 mm, 2.1 μm), acetonitrile and 0.05% phosphoric acid as the mobile phase gradient elution; detection wavelength 350nm, flow rate 1.0 ml · min-1, the column temperature was 45 ℃. the results of luteolin in 0.021 48 1.074 μg was linear within the range (r = 0.999 9), recovery was 98.45% (n = 6), RSD = 1.21%; hyperin in 0.020 48 ~ 1.024 μg (r = 0.999 9) within showed a linear relationship, recovery was 96.77% (n = 6), RSD = 0.56%; chlorogenic acid in 0.202 1 ~ 10.104 μg was linear within the range (r = 0.999 6), recovery was 99.12% (n = 6), RSD = 0.87%. Conclusion The analysis time is short, accurate, reproducible, and can be used for quality control of honeysuckle. [Key Words] high-speed liquid chromatography; honeysuckle; luteolin; hyperin; chlorogenic acid Honeysuckle honeysuckle plants are honeysuckle Lonicera japonica Thunb. With dry buds or open flowers, cold, sweet, the lung, heart and stomach. With detoxification, the effect of wind and heat evacuation, for the treatment of carbuncle swollen boils, Houbi, erysipelas, dysentery Noxious Heat, Fengreganmao, febrile disease fever embolism [1]. honeysuckle in the more complex ingredients, including organic acids, flavonoids, triterpenoid saponins and volatile oils. At present, for the determination of honeysuckle herbs concentrated in the HPLC method for the determination of chlorogenic acid and luteolin [2,3], but the analysis time is long, poor separation. ultra-high speed liquid chromatography (UFLC ) Shimadzu Corporation, Japan launched nearly two years a new type of liquid chromatography, which uses special technology to make the analysis time much shorter, and can effectively sav