Honeysuckle flower extract sperm injection with crude extracts of active systemic allergic Study.docVIP

 PAGE \* MERGEFORMAT 14 Honeysuckle flower extract sperm injection with crude extracts of active systemic allergic Study Authors: Feng Wenyu Ming-Hua Liu Shun-Han Xiao Bing [Abstract] Objective Extraction and Purification of different extracts prepared honeysuckle immunization injection toxicity test in order to understand with the injection-induced allergy Honeysuckle reasons. Method according to ‘traditional Chinese medicine, natural medicine, immune toxicity studies of technical guidelines’ requirements, do honeysuckle extract sperm injection with crude extract of active systemic allergy test. The amount of guinea pig sensitized by intraperitoneal injection of chlorogenic acid into account, respectively 7.5,1.5 mg * kg-1, intravenous injection of excitation volume is two times the amount of allergens stimulate the detailed observations recorded after the reaction of each animal. Results honeysuckle extract sperm injection high-and low-dose group animals were not an allergic reaction, while the crude extract of honeysuckle injection of high-dose group appeared strong positive reaction type allergies and low-dose group appeared positive. Conclusion honeysuckle crude extract injection type allergic reactions occur because, with the injection fluid contains protein, resin, tannins and other impurities on. [Keywords:] Honeysuckle refined extract; honeysuckle crude extract; injection; chlorogenic acid; allergy test Abstract: ObjectiveTo study the immune toxicity of Honeysuckle extract injection prepared by different extraction and purification processes, in order to analyze the causes of allergic reaction caused by Honeysuckle injection. MethodsAccording to the requirement of amp;quot;Chinese traditional medicine, natural medicine immunity toxicity study rulleramp;quot; , we studied active systematic anaphylaxis of Honeysuckle refined extract and crude extract injection. Sensitizing dose (ip) counted as chlorogenic acid was 7.5,1.5 mg * kg-1, excitation dose