Histopathological technique frequently used in sports science and the application of the human body.docVIP

 PAGE \* MERGEFORMAT 7 Histopathological technique frequently used in sports science and the application of the human body [Paper Keywords] Histopathological techniques Human Movement Science [Abstract] In recent years molecular biology techniques, as well as other high and new technology in the field of pathology is widely used, the same as a basic research studies sports science and sports development of the human body can no longer satisfy the simple macro research, more and more medical examination and the pathology of human science experimental techniques widely used in sports, especially histopathology technology positioning technologies, such as immunohistochemistry, in situ hybridization. In this paper, the above three kinds of pathology commonly used method of positioning technology are reviewed. An experimental technique immunohistochemistry With immunohistochemical staining techniques widely used, pathological study brings landmark leap forward. Immunohistochemistry is a method commonly used in experimental pathology, is at the protein level in various cell-specific antibodies against various antigens. According to the different markers, immunohistochemical techniques can be divided into immunofluorescent cytochemistry, immunological enzyme cytochemistry, immunology ferritin techniques, immuno-gold - silver-cell chemical technology, pro-and immunocytochemical techniques, immune electron microscopy techniques . 1.1 Immunohistochemistry step immunohistochemical staining technique is a combination of the methods of immunology theory and technology developed a new technology. The principle is to use the core of immunology - the principle of antigen-specific binding body with labeled antibody to track antigen, through chemical reaction marked on the combination of specific antibodies displayed on the agent, such as enzymes, metal ions, isotopes and other display a certain color, and the aid of a microscope, fluorescence microscope, electron micro