Histone deacetylase inhibitor SAHA blocked MAPK signal pathway and induce apoptosis in HL 60.docVIP

 PAGE \* MERGEFORMAT 27 Histone deacetylase inhibitor SAHA blocked MAPK signal pathway and induce apoptosis in HL 60 Author: Wang Ying Wang Yu HOU Chunmei Xu Yuan Chi Yan Yu Xiao-Dan Chidu Abstract This study aims to explore the histone deacetylase inhibitor-SAHA (suberoylanilide hydroxamic acid)-induced acute myeloid leukemia cell line HL  60 Apoptosis in the molecular mechanism. Using MTT method, Wright  Hoechst33342/PI Giemsa staining and fluorescent staining methods to detect SAHA right HL  60 cell growth inhibition and cell morphological changes; by flow cytometry, Western  blot was determined HL  60 cell cycle changes and a variety of signaling protein expression. The results showed that: SAHA can be dose-time-dependent inhibition of the growth of HL  60 cells; by 2 μmol / L SAHA treatment 12-48 hours, HL  60 cells showed that the cell cycle was arrested in G0/G1 phase; by Hoechst33342 staining apparent after the nuclei of apoptotic body structure; Western blot test confirmed that, SAHA role of the HL  60 cells activated caspase 3 and its substrate protein, poly-ADP-ribose polymerase [poly (ADP  ribose) polymerase, PARP] occurred scissors cutting, cell apoptosis; on apoptosis-related signal transduction pathway P44/42 MAPK and PI3K/Akt test results show that MAPK signaling pathway involving Raf and ERK kinase phosphorylation was inhibited, while the Akt and its downstream mTOR kinase activity did not change significantly. Conclusions: SAHA right HL  60 cells cytotoxicity is mainly achieved through the induction of apoptosis, and cell proliferation-related P44/42 MAPK signal pathway is blocked is one of the mechanisms of apoptosis occurred. Keywords: histone deacetylase inhibitors; SAHA; MAPK; signal transduction; apoptosis; HL  60 cells, Histone Deacetylase Inhibitor SAHA Induces Inactivation of MAPK Signaling and Apoptosis in HL  60 Cells Abstract The study was aimed to investigate the molecular mechanisms of