HPLC determination of glycyrrhizic acid in Glycyrrhiza inflata content.docVIP

 PAGE \* MERGEFORMAT 11 HPLC determination of glycyrrhizic acid in Glycyrrhiza inflata content Keywords: liquorice Licorice licorice for legumes (glycyrrhiza uralensis fisch), Glycyrrhiza inflata (glycyrrhiza inflata bat), or Glycyrrhiza glabra (glycyrrhiza glabra l) of the dried root and rhizome, only contained in the “after” a long medicinal history. Its function Spleen Qi, lungs cough, urgency and pain relief, Qingrejiedu to ease the effects of the medicine. Main containing glycyrrhizic acid and licorice glycosides, in which glycyrrhizin application of modern pharmacological studies confirmed with anti-fibrosis, anti-inflammatory, anti-virus and Liver detoxification and strengthen the role of immune function [1]. At present the internal quality control for the licorice, glycyrrhizic acid composition mainly focus on qualitative and quantitative examination of a class. 2005 edition of “Chinese Pharmacopoeia” reproduces ternary isocratic RP-HPLC Determination of glycyrrhizic acid elution method, the provisions of glycyrrhizic acid content shall not be less than 2% [2]. In this study, relying on 2005 edition of “Chinese Pharmacopoeia” a determination of glycyrrhizic acid content (Appendix VID) operate under the main line, using RP-HPLC determination of glycyrrhizic acid content, method is simple, rapid and reproducible results are stable for glycyrrhizic acid test specific, An instrument and reagent 1.1 Instrument Agilend 1100 Series liquid chromatograph, quaternary pump, on-line vacuum degasser, DAD detector, ChemStation, 1200 autosampler. 1.2 Reagents Monoammonium Glycyrrhizinate (monoam0nium glycyrrhitinate) reference substance (Chinese medicines and biological products); liquorice (glycyrrhizain flata bat) sample, origin of Xinjiang (East China Pharmaceutical Co., Ltd.); methanol (HPLC pure), ammonium acetate (analytical pure), glacial acetic acid (AR), water (double distilled water). 2 Experimental Methods