HPLC Determination of matrine Zhi Li Ning Tablet.docVIP

 PAGE \* MERGEFORMAT 9 HPLC Determination of matrine Zhi Li Ning Tablet [Abstract] Objective To establish a high-performance liquid chromatography (HPLC) Determination of matrine Zhi Li Ning tablets. Methods The C18 column (150mm * 4.6 mm, 5 μ m), with acetonitrile - water (1 000 ml of water plus 2 ml triethylamine, pH, adjusted with phosphoric acid to 3.0) (four ninety-six) as mobile phase, flow rate of 1.0 ml / min, column temperature of 40 ℃ , detection wavelength was 220 nm. The results in the establishment of the chromatographic conditions, matrine and impurity can be completely separated from the linear range of matrine 18.712 ~ 299.392 μ g / ml (r = 0.999 9), the average recovery was 101.60%, RSD was 0.8%. Conclusion This method is simple, accurate, specific and well, Zhi Li Ning can be used for determination of matrine in the film. [Keywords:] Zhi Li Ning-chip high-performance liquid chromatography of matrine Zhi Li Ning film is by the CSC, Sophora flavescens, costas composed of semi-extract tablets, with a heat dampness, qi pain and other effects [1], Andrographis paniculata, and Sophora are one of the main drug. This product has since been contained in the Ministry of Health, ‘the standard of Chinese medicine set prescription drug formulations’ fourth book, not its quality standards determination of entry. China has been on the drug in dehydroandrographolide Determination of studies have reported, but no reports on the determination of matrine in order to better control the quality of films Zhi Li Ning, the research group studying the establishment of the HPLC Method preparation of the content of matrine method is simple, accurate, specific and well, can effectively control the preparation of the inherent quality. An apparatus and materials HPLC: Agilent 1200 (with the Agilent ChemStation); one hundred thousandth electronic balance (Sartorius cp225D). Matrine reference substance (Lot No. :110805-200306, for determination purposes), and by the Chi