HPLC determination of plasma concentrations of nevirapine.docVIP

 PAGE \* MERGEFORMAT 5 HPLC determination of plasma concentrations of nevirapine [Keywords:] chromatography, high pressure liquid; nevirapine; blood drug concentration [Keywords:] chromatography, high pressure liquid; nevirapine; blood drug concentration Nevirapine (nevirapine) is a human immunodeficiency virus (HIV1) of non-nucleoside reverse transcriptase inhibitor (NNRTI). Nevirapine and HIV1 reverse transcriptase (RT) to make this direct connection and through the catalytic enzyme to block the RNA-side fault dependent and DNA-dependent DNA polymerase activity [1-2]. nevirapine is not with the nucleoside triphosphate substrate, or competition as well. Our aim is to explore a simple, rapid, specific determination of high nevirapine plasma concentrations approach. 1 Materials and methods 1.1 Materials Agilent 1100 liquid chromatograph (Agilent Technologies U.S. production); low-temperature high-speed centrifuge (Germany SIGMA company); oscillator (U.S. Keerpamo Instruments production); ten thousandth scale (Shanghai Instrument Factory production of equity instruments ). methanol, acetonitrile, chromatographic pure, Merck’s production; phosphoric acid, sodium dihydrogen phosphate, trichloroacetic acid, triethylamine, AR grade, Shanghai Chemical Reagent Company production. Subjects agents: Domestic nevirapine capsules (trade name Ai pole ), lot number 0309001, each pill is 200 mg, Zhejiang Huahai Pharmaceutical Co., Ltd. production; domestic nevirapine tablets (trade name Ai too), lot number 0402004, per tablet 200 mg, Zhejiang Huahai Pharmaceutical Co., Ltd. production; Participation than the formulations: import nevirapine tablets (brand name vitamin A), batch number 304185d, per tablet 200 mg, Boehringer Ingelheim production; nevirapine standard, batch number 556,503,002, content 99.8%, Zhejiang Huahai Pharmaceutical Co., Ltd. to provide . 1.2 Methods 1.2.1 The chromatographic conditions were column: Hypersil C18 column (150 mm * 4.6 mm, 0.5 μ m); mobi