HPLC determination of puerarin in Xuanfei Pills content.docVIP

 PAGE \* MERGEFORMAT 12 HPLC determination of puerarin in Xuanfei Pills content 【Abstract】 Objective To determine the Xuanfei Pill method for the determination of puerarin. High-performance liquid chromatography method using ZORBAX SB  C18 (250 mm × 4.6 mm, 5 μm) column, mobile phase: methanol - water (22:78), flow rate: 1.0 ml * min  1, detection wavelength 250 nm, column temperature 40 ℃, injection volume 10 μl. RESULTS The linear range is: Puerarin 0.0463 2 ~ 0.694 8 μg, r = 1.0. The average recovery was 103.24%, RSD 0.44%. Conclusion The separation of good, fast and simple, can be used as the product quality control methods. Keywords: Xuanfei pill; puerarin; high performance liquid chromatography Abstract: ObjectiveTo establish a method for determination of puerarin in Xuanfeipill. MethodsHPLC was used for quantitative ananlysis. The ZORBAX SB-C18 (250 mm × 4.6mm, 5μm) column was used, and the mobile phase was composed of methanol-water (22 : 78). Detection wavelength was at 250 nm, the flow rate was 1.0 ml * min-1, column temperature was at 40 ℃, and the injection volume was 10 μl. ResultsThe linear response was in the range of 0.046 32 ~ 0.694 8 μg puerarin (r = 1.0). The average recovery of puerarin was 103.24%, RSD was 0.44%. ConclusionThe method is simple, accurate, and it is suitable for the determination of the purarin in compound preparations. Keywords:: Xuanfeiwan; Puerarin; HPLC Xuanfei pills from Pueraria lobata, ginseng and many other Chinese herbal medicines composed of compound preparation, in order to close ministerial section contains seven species, with a solution of the table cold-dispelling, phlegm effect Xuanfei. For lung phlegm, cold headache, stuffy nose caused by cold, whole body discomfort, cough, phlegm, Xiongge full nausea, gas, nausea, etc. against the card. Kudzu has a fever Jie Ji, Sheng Jin efficacy of drugs for the Fang-jun, the main ingredients of puerarin, flavonoids [1], the origin