HPLC determination of OsteoKing hesperidin tablets.docVIP

 PAGE \* MERGEFORMAT 8 HPLC determination of OsteoKing hesperidin tablets [Abstract] Objective To establish OsteoKing to test the content of hesperidin tablets. Methods using high performance liquid chromatography, Shim-pack-C18 column (150 mm × 4.6 mm, 5 μm), acetonitrile -0.4 % phosphoric acid (20:80) as mobile phase, detection wavelength 283 nm. the results of the linear range of hesperidin 0.194 ~ 0.970 μg, r = 0.999 9, the average recovery of hesperidin was 98.50%, RSD = 0.63% . Conclusions The method used to determine the film OsteoKing hesperidin, a simple, accurate, sensitive, and can be used as indicators of the quality control of the preparation. [Keywords:] OsteoKing film, hesperidin, HPLC, determination Abstract: Objective To determine the content of Hesperidin in Henggu Gushangyu Tablets by high performance liquid chromatography. Methods The determination was conducted on Shim-pack-C18 column (150 mm × 4.6 mm, 5 μm) with mobile phase of acetonitrile-0.4% phosphoric acid water (20:80). The detection wavelength was 283 nm. Results Good linear relationship of Hesperidin between area and amount was noted for 0.194 ~ 0.970 μg, with a correlation coefficient of 0.999 9. The average recovery was 98.50%, and RSD was 0.63%. Conclusion The established method is simple, accurate and sensitive, and can be applied to the quality control of the preparation. Keywords:: Henggu Gushangyu Tablets, Hesperidin, HPLC, content determination OsteoKing film is constant by the medicine of ancient bone healing agent (closing contained in the lt;”State standards for compilation of orthopedic medicine Volumegt;gt;) made changes in formulations and new agents [1], Smell the orange peel, safflower, Panax composed of 9 herbs, Qi with blood, liver or kidney, bone continued reinforcement, swelling and pain, promote healing effect for fresh fractures and delayed fractures and femoral head necrosis, bone joint disease, lumbar disc psychosis. to control the quality o