HPLC determination of puerarin in cold Qingre particle content.docVIP

 PAGE \* MERGEFORMAT 6 HPLC determination of puerarin in cold Qingre particle content [Abstract] Objective cold Qingre HPLC method for particle determination of puerarin. Methods Hypersil C18 column (5μ m, 100mm * 4.6mm), methanol - water (22:78) as mobile phase, detection wavelength 250nm, flow rate 1.0ml/min. The results of the linear range of 0.1384 ~ 1.384μ g (r = 0.9997), the average recovery was 99.84%, RSD = 1.65%. Conclusion The method is simple, sensitive and accurate. [Keywords:] HPLC cold Qingre granules puerarin [Abstract] Objective To determine content of puerarin in Ganmaoqingre granule by HPLC.Methods Hypersil C18 (5μ m, 100 * 4.6mm) was used, and the Meoh-eater (22:78) was served as mobile phase, and it’s flowrate was 1.0ml/min, wave length of determining was 250nm.Results The linearity range was within 0.1384 ~ 1.384μ g (r = 0.9997). The average recovery was 99.84%, RSD = 1.65%. Conclusion This method is simple, sensitive, and accurative. [Keywords:] HPLC; Ganmaoqingre Granule; Puerarin Cold Qingre particles from Nepeta ear, bitter to Ding, puerarin of 11 Chinese herbal medicines are made with wind-dispersing and cold-dispelling, Relieve the effect of heat, for cold cold, headache fever, body pain, aversion to cold, clear tears streaming nose, cough, pharyngeal dry. In order to better control product quality, this study HPLC method for its use of the active ingredient Pueraria Chen drugs for determination of puerarin with satisfactory results. An instrument and reagent Agilent 1100 HPLC: quaternary gradient pump, diode array detector, Agilent 1100 workstation; TP-150 ultrasonic cleaner, AG135 Electronic Analytical Balance; puerarin reference substance (110752-200108, for use with measurement) from Chinese medicines and biological products available; cold Qingre particles provided by the Hunan Pharmaceutical Co., Ltd.; methanol HPLC pure water to ultrapure water, other reagents were analytical pure. 2 Experimental Methods and Results